Bouffier L., Yiu H. H. P., Rosseinsky M. J.
Langmuir 2011, 27, 6185-6192.

Spherical magnetite nanoparticles (MNPs, 24 nm in diameter) were sequentially functionalized with trimethoxysilylpropyldiethylenetriamine (TMSPDT) and a synthetic DNA intercalator, namely, 9-chloro-4H-pyrido[4,3,2-kl]acridin-4-one (PyAcr), in order to promote DNA interaction. The designed synthetic pathway allowed control of the chemical grafting efficiency to access MNPs either partially or fully functionalized with the intercalator moiety. The newly prepared nanomaterials were characterized by a range of physicochemical techniques: FTIR, TEM, PXRD, and TGA. The data were consistent with a full surface coverage by immobilized silylpropyldiethylenetriamine (SPDT) molecules, which corresponds to 22300 SPDT molecules per MNP and a subsequent (4740 − 2940) PyAcr after the chemical grafting step (i.e., 2.4 PyAcr/nm2). A greater amount of PyAcr (30600) was immobilized by the alternative strategy of binding a fully prefunctionalized shell to the MNPs with up to 16.1 PyAcr/nm2. We found that the extent of PyAcr functionalization strongly affects the resulting properties and, particularly, the colloidal stability as well as the surface charge estimated by ζ-potential measurement. The intercalator grafting generates a negative charge contribution which counterbalances the positive charge of the single SPDT shell. The DNA binding capability was measured by titration assay and increases from 15 to 21.5 μg of DNA per mg of MNPs after PyAcr grafting (14−20% yield) but then drops to only 2 μg for the fully functionalized MNPs. This highlights that even if the size of the MNPs is obviously a determining factor to promote surface DNA interaction, it is not the only limiting parameter, as the mode of binding and the interfacial charge density are essential to improve loading capability.

 

 

Surface Compaction Versus Stretching in Pickering Emulsions Stabilised by Microgels
Thursday, 07 November 2013
Schmitt V., Ravaine V.,Curr. Opin. Colloid Interface Sci. 2013, 18, 532–541. Review Colloidal gel particles called microgels have shown their ability to adsorb at an oil–water interface and...
Read More...
Electrokinetic Assembly of One-Dimensional Nanoparticle Chains with Cucurbit[7]uril Controlled Subnanometer Junctions
Friday, 01 November 2013
Husken N., Taylor R. W., Zigah D., Taveau J.-C., Lambert O., Scherman O. A., Baumberg J. J., Kuhn A.,Nano Lett. 2013, 13, 6016–6022. One-dimensional (1D) nanoparticle chains with defined...
Read More...
Photochemical Functionalisation of Optical Nanotips with a Rhodamine Chemosensor for Remote Through-Fiber Detection of Hg2+
Monday, 21 October 2013
Chen K., Adam C., Sojic N., Schmittel M.,RSC Adv. 2013, 33, 24140–24145. For remote Hg2+ detection, an array of nanotips was covalently functionalised using a versatile photochemical approach....
Read More...
Pickering Emulsions Stabilized by Soft Microgels: Influence of the Emulsification Process on Particle Interfacial Organization and Emulsion Properties
Thursday, 19 September 2013
Destribats M., Wolfs M., Pinaud F., Lapeyre V., Sellier E., Schmitt V., Ravaine V.,Langmuir 2013, 29, 12367–12374. This work reports a new evidence of the versatility of soft responsive microgels...
Read More...
Electrochemical Detection of Single Microbeads Manipulated by Optical Tweezers in the Vicinity of Ultramicroelectrodes
Tuesday, 10 September 2013
Suraniti E., Kanoufi F., Gosse C., Zhao X., Dimova R., Pouligny B., Sojic N.,Anal. Chem. 2013, 85, 8902–8909. Editors' Highlight ! Latex micrometric beads are manipulated by optical tweezers in the...
Read More...
Comprehensive Study of DNA Binding on Iron(II,III) Oxide Nanoparticles with a Positively Charged Polyamine Three-Dimensional Coating
Tuesday, 13 August 2013
Yiu H. H. P., Bouffier L., Boldrin P., Long J., Claridge J. B., Rosseinsky M. J.,Langmuir 2013, 29, 11354-11365. Iron (II,III) oxide Fe3O4 nanoparticles (25 and 50 nm NPs) are grafted with amine...
Read More...
Capillary Electrophoresis as a Production Tool for Asymmetric Microhybrids
Wednesday, 17 July 2013
Fattah Z., Garrigue P., Goudeau B., Lapeyre V., Kuhn A., Bouffier L.,Electrophoresis 2013, 34, 1985-1990. The site selective electrodeposition of silver metal onto a conducting object such as carbon...
Read More...
Development of Electrochemical Microsensors for the Monitoring of Mitochondrial Activities
Tuesday, 18 June 2013
Vanhove E., Ben-Amor S., Charlot S., Colin D., Devin A., Rigoulet M., Sojic N., Sékli Belaïdi F., Launay J., Temple-Boyer P., Arbault S.,I.E.E.E., Proceedings of Transducers 2013, 1135-1138....
Read More...
Bipolar Electrochemistry: From Materials Science to Motion and Beyond
Wednesday, 29 May 2013
Loget G., Zigah D., Bouffier L., Sojic N., Kuhn A.Acc. Chem. Res. 2013, 46, 2513-2523. Bipolar electrochemistry, a phenomenon which generates an asymmetric reactivity onthe surface of conductive...
Electrochemiluminescent Polymer Film with suitable Redox “turn-off” Absorbance window for Remote Selective Sensing of Hg2+
Thursday, 16 May 2013
Shu Q., Adam C., Sojic N., Schmittel M.,Analyst 2013, 138, 4500-4504. An electrochemiluminescent sensing scheme is presented for remote detection of Hg2+ through an ITO-coated nanostructured optical...
Read More...
Photochemical Crosslinking of Hyaluronic Acid confined in Nanoemulsions: towards Nanogels with a Controlled Structure
Tuesday, 07 May 2013
Messager L., Portecop N., Hachet E., Lapeyre V., Pignot-Paintrand I., Catargi B., Auzély-Velty R., Ravaine V.,J. Mater. Chem. B 2013, 1, 3369-3379. We present the preparation of nanogels made of...
Read More...
Monitoring Metabolic Responses of Single Mitochondria within PDMS Wells: Study of their Endogenous NADH Evolution
Friday, 19 April 2013
Suraniti E., Vajrala S. R., Goudeau B., Bottari S., Rigoulet M., Devin A., Sojic N., Arbault S.,Anal. Chem. 2013, 85, 5146-5152. It is now demonstrated that mitochondria individually function...
Read More...
Functionalization of Optical Nanotip Arrays with an Electrochemical Microcantilever for Multiplexed DNA Detection
Friday, 12 April 2013
Descamps E., Duroure N., Deiss F., Leichlé T., Adam C., Mailley P., Aït-Ikhlef A., Livache T., Nicu L., Sojic N.,Lab on a Chip 2013, 13, 2956-2962. Optical nanotip arrays fabricated on etched fiber...
Read More...
Enhanced Electrogenerated Chemiluminescence in Thermo-Responsive Microgels
Friday, 29 March 2013
Pinaud F., Russo L., Pinet S., Gosse I., Ravaine V., Sojic N.,J. Am. Chem. Soc. 2013, 135, 5517-5520. The electrochemistry, photoluminescence and electrogenerated chemiluminescence of...
Read More...
Chemiluminescence from Asymmetric Inorganic Surface Layers generated by Bipolar Electrochemistry
Thursday, 07 March 2013
Fattah Z., Roche J., Garrigue P., Zigah D., Bouffier L., Kuhn A.,Chem. Phys. Chem. 2013, 14, 2089-2093. Blue light on a Janus rod: Prussian Blue (PB) is locally generated in an asymmetric way on a...
Read More...
Introducing a well-ordered Volume Porosity in 3-Dimensional Gold Microcantilevers
Monday, 04 February 2013
Ayela C., Lalo H., Kuhn A.,Appl. Phys.Lett. 2013, 102, 053501. The purpose of the present work is the introduction of a combined bottom-up and top-down approach to generate 3-dimensional gold...
Read More...
Enhanced Hydrogen Peroxide Sensing based on Prussian Blue modified Macroporous Microelectrodes
Sunday, 27 January 2013
Mokrushina A. V., Heim M., Karyakina E. E., Kuhn A., Karyakin A. A.,Electrochem. Comm. 2013, 29, 78-80. We report on the successful modification of macroporous microelectrodes with the...
Read More...
Oxygen Plasma Treatment Of Platinized Ultramicroelectrodes Increases Sensitivity For Hydrogen Peroxide Detection On Mitochondria
Thursday, 24 January 2013
Ben-Amor S., Devin A., Rigoulet M., Sojic N., Arbault S., Electroanalysis 2013, 25, 656-663. Hydrogen peroxide is a major bio-active molecule produced by mitochondria during aerobic metabolism. In...
Read More...
Wireless Electrografting of Molecular Layers for Janus Particle Synthesis
Friday, 28 December 2012
Kumsapaya C., Bakai M.-F., Loget G., Goudeau B., Warakulwit C., Limtrakul J., Kuhn A., Zigah D.,Chemistry - A European Journal 2013, 19, 1577-1580. Glassy carbon beads are selectively functionalized...
Read More...
Hierarchical Macro-mesoporous Pt Deposits on Gold Microwires for Efficient Methanol Oxidation
Monday, 05 November 2012
Heim M., Wattanakit C., Reculusa S., Warakulwit C., Limtrakul J., Ravaine S., Kuhn A.,Electroanalysis 2013, 25, 888-894. Hierarchically ordered macro-mesoporous Pt films have been synthesized on gold...
Read More...
Design of a Wireless Electrochemical Valve
Thursday, 01 November 2012
Bouffier L., Kuhn A., Nanoscale 2013, 5, 1305-1309. The control of motion of small objects is an emerging research area. Their design strongly depends on the strategy chosen to trigger the...
Read More...
Effect of Novel Antibacterial Gallium-Carboxymethyl Cellulose on Pseudomonas Aeruginosa
Tuesday, 30 October 2012
Valappil S. P., Yiu H. H. P., Bouffier L., Hope C. K., Evans G., Claridge J. B., Higham S. M., Rosseinsky M. J., Dalton Trans. 2013, 42, 1778-1786. Gallium has emerged as a new therapeutic agent due...
Read More...
Interest of the Sol-Gel Approach for Multiscale Tailoring of Porous Bioelectrode Surfaces
Friday, 26 October 2012
Bon Saint Côme Y., Lalo H., Wang Z., Kohring G.W, Hempelmann R., Etienne M., Walcarius A., Kuhn A., Electroanalysis 2013, 25, 621-629. Gold nanoparticles have been co-encapsulated with d-sorbitol...
Read More...