E. Pedraza, A. Karajić, M. Raoux, R. Perrier, A. Pirog, F. Lebreton, S. Arbault, J. Gaitan, S. Renaud, A. Kuhn, J. Lang
Lab Chip, 2015, 15, 3880–3890
We are developing a cell-based bioelectronic glucose sensor that exploits the multi-parametric sensing
ability of pancreatic islet cells for the treatment of diabetes. These cells sense changes in the concentration
of glucose and physiological hormones and immediately react by generating electrical signals. In our sensor,
signals from multiple cells are recorded as field potentials by a micro-electrode array (MEA). Thus, cell
response to various factors can be assessed rapidly and with high throughput. However, signal quality and
consequently overall sensor performance rely critically on close cell–electrode proximity. Therefore, we
present here a non-invasive method of further exploiting the electrical properties of these cells to guide
them towards multiple micro-electrodes via electrophoresis. Parameters were optimized by measuring the
cell's zeta potential and modeling the electric field distribution. Clonal and primary mouse or human
β-cells migrated directly to target electrodes during the application of a 1 V potential between MEA
electrodes for 3 minutes. The morphology, insulin secretion, and electrophysiological characteristics were
not altered compared to controls. Thus, cell manipulation on standard MEAs was achieved without introducing
any external components and while maintaining the performance of the biosensor. Since the analysis
of the cells' electrical activity was performed in real time via on-chip recording and processing, this work
demonstrates that our biosensor is operational from the first step of electrically guiding cells to the final
step of automatic recognition. Our favorable results with pancreatic islets, which are highly sensitive and
fragile cells, are encouraging for the extension of this technique to other cell types and microarray devices.
Indirect Bipolar Electrodeposition Tuesday, 27 November 2012
Loget G., Roche J., Gianessi E., Bouffier L., Kuhn A.,J. Am. Chem.Soc. 2012, 134, 20033-20036. Based on the principles of bipolar electrochemistry, localized pH gradients are generated at the surface... Read More...
Light-Emitting Electrochemical Swimmers Monday, 08 October 2012
Sentic M., Loget G., Manojlovic D., Kuhn A., Sojic N.,Angew. Chem. Int. Ed. 2012, 51, 11284–11288. Swimmer in the dark: Propulsion of a conducting object is intrinsically coupled with light... Read More...
Development of Functionalized Cyclotriveratrylene Analogues: Introduction of Withdrawing and p-Conjugated Groups Monday, 30 July 2012
Peyrard L., Dumartin M.-L., Chierici S., Pinet S., Jonusauskas G., Meyrand P., Gosse I.,J. Org. Chem. 2012, 77, 7023-7027. Cyclotriveratrylene analogues (CTVs) are supramolecular bowl-shaped... Read More...
Direct Visualization of Symmetry Breaking during Janus Nanoparticle Formation Tuesday, 03 July 2012
Loget G., Lee T.-C., Taylor R. W., Mahajan S., Nicoletti O., Jones S. T., Coulston R. J., Lapeyre V., Garrigue P., Midgley P. A., Scherman O. A., Baumberg J. J., Kuhn A.,Small 2012, 8, 2698-2703. The... Read More...
Combined Macro/mesoporous Microelectrode Arrays (MEAs) for Low Noise Extracellular Recording of Neural Networks Wednesday, 27 June 2012
Heim M., Rousseau L., Reculusa S., Urbanova V., Mazzocco C., Joucla S., Bouffier L., Vytras K., Bartlett P., Kuhn A., Yvert B.Journal of Neurophysiology 2012, 15, 1793-1803. Microelectrode arrays... Read More...
The Fraction of Alpha-linolenic Acid present in the sn-2 position of Structured Triacylglycerols decreases in Lymph Chylomicrons and Plasma Triacylglycerols during the Course of Lipid Absorption in Rats Wednesday, 30 November 2011
Couedelo L., Vaysse C., Vaique E., Guy A., Gosse I., Durand T., Pinet S., Cansell M., Combe N.Journal of Nutrition 2012, 142, 70-75. Little is known about the ability of α-linolenic acid (Ln) to...