A. de Poulpiquet, B. Diez-Buitrago, M. Dumont Milutinovic, M. Sentic, S. Arbault, L. Bouffier, A. Kuhn, N. Sojic

Anal. Chem., 201688, 6585–6592

The combination of enzymes, as recognition elements for specific analytes, and of electrogenerated chemiluminescence (ECL) as a readout method has proven to be a valuable strategy for sensitive and specific analytical detection. However, ECL is intrinsically a 2D process which could potentially limit the analysis of inhomogeneous samples. Here we show how a bulk ECL signal, generated by thousands of carbon microbeads remotely addressed via bipolar electrochemistry, are implemented as a powerful tool for the concomitant ECL sensing and imaging of two enzymatic substrates. We selected two enzymes (glucose dehydrogenase and choline oxidase) that react with their respective model substrates and produce in situ chemical species (NADH and H2O2) acting as co-reactants for the ECL emission of different luminophores ([Ru(bpy)3]2+ at l = 620 nm and luminol at l = 425 nm, respectively). Both enzymes are spatially separated in the same capillary. We demonstrate thus the simultaneous quantitative determination of both glucose and choline over a wide concentration range. The originality of this remote approach is to provide a global chemical view through one single ECL image of inhomogeneous samples such as a biochemical concentration gradient in a capillary configuration. Finally, we report the first proof-of-concept of dual biosensing based on this bulk ECL method for the simultaneous imaging of both enzymatic analytes at distinct wavelengths.

2016-12

Clogging by sieving in microchannels: application to the detection of contaminants in colloidal suspensions
Friday, 08 April 2016
A. Sauret, E. C. Barney, A. Perro, E. Villermaux, H. A. Stone and E. Dressaire  Applied Physics Letters 2014, 105, 074101  We report on a microfluidic method that...
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Readily Prepared Dynamic Hydrogels by Combining Phenyl Boronic Acid- and Maltose-Modified Anionic Polysaccharides at Neutral pH
Friday, 19 December 2014
D. Tarus, E. Hachet, L. Messager, B. Catargi, V. Ravaine, R. Auzély-Velty Macromol. Rapid. Commun. 2014, 35, 2089 -2095 Dynamic covalent hydrogels are facilely prepared from biocompatible...
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Impact of Electrostatics on the Adsorption of Microgels at the Interface of Pickering Emulsions
Friday, 19 December 2014
P. Masse, E. Sellier, V. Schmitt, and V. Ravaine Langmuir, 2014, 30 (49), pp 14745–14756 The importance of electrostatics on microgel adsorption at a liquid interface is studied, as well as its...
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Wireless Electrosampling of Heavy Metals for Stripping Analysis with Bismuth-Based Janus Particles
Wednesday, 19 November 2014
H. Sopha, J. Roche, I. Švancara, A. Kuhn Anal.Chem. 2014, 86, 10515–10519 In this contribution, a wireless method for the electrolytic sampling of heavy metals at special bismuth-modified...
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Wireless powering of e-swimmers
Tuesday, 21 October 2014
J. Roche, S. Carrara, J. Sanchez, J. Lannelongue, G. Loget, L. Bouffier, P. Fischer, A. Kuhn Sci. Rep. 2014, 4, 6705 Miniaturized structures that can move in a controlled way in solution and...
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Stimuli-responsive microgels for electrochemiluminescence amplification
Tuesday, 07 October 2014
F. Pinaud, N. Sojic, V. Ravaine L'Actualité Chimique, 2014, 390. In the present work, redox thermoresponsive microgels bearing ruthenium complex were synthesized. Investigation of electrogenerated...
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Physico-chemical Milling for controlled Size Reduction of Metal Beads
Thursday, 28 August 2014
J. Roche, E. Gianessi, A. Kuhn Phys. Chem. Chem. Phys. 2014, 16, 21234-21236. Here we report a new physico-chemical method based on bipolar electrochemistry for producing spherical metal beads with a...
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Asymmetric Modification of TiO2 Nanofibers with Gold by Electric-Field-Assisted Photochemistry
Thursday, 31 July 2014
M. Ongaro, J. Roche, A. Kuhn, P. UgoChem. Electro. Chem. 2014, 2048-2051 We present a new procedure for the site-specific deposition of metal on semiconductor nanofibers (NF). The semiconductor NFs...
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Electrochemiluminescent Swimmers for Dynamic Enzymatic Sensing
Monday, 30 June 2014
M. Sentic, S. Arbault, B. Goudeau, D. Manojlovic, A. Kuhn, L. Bouffier, N. SojicChem. Commun. 2014, 50, 10202-10205. An electrochemiluminescent (ECL) swimmer driven by bipolar electrochemistry is...
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Monitoring Electrochemically the Early Events of Hydrogen peroxide Production by Mitochondria
Thursday, 22 May 2014
E. Suraniti, S. Ben-Amor, P. Landry, M. Rigoulet, E. Fontaine, S. Bottari, A. Devin, N. Sojic, N. Mano, S. ArbaultAngew. Chem. Int. Ed. 2014, 126, 6773-6776.Mitochondria consume oxygen at the...
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Electropolymerization of Polypyrrole by Bipolar Electrochemistry in an Ionic Liquid
Tuesday, 13 May 2014
S. Kong, O. Fontaine, J. Roche, L. Bouffier, A. Kuhn, D. ZigahLangmuir 2014, 30, 2973−2976 Bipolar electrochemistry has been recently explored for the modification of conducting micro- and...
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Adsorption of Microgels at an Oil–Water Interface: Correlation between Packing and 2D Elasticity
Friday, 25 April 2014
F. Pinaud, K. Geisel, P. Massé, B. Catargi, L. Isa, W. Richtering, V. Ravaine, V. SchmittSoft Matter, 2014, in press. The aim of this paper is to determine how microgels adsorb at a model...
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Imaging Redox Activity at Bipolar Electrodes by Indirect Fluorescence Modulation
Friday, 21 March 2014
Bouffier L., Doneux T., Goudeau B., Kuhn A.Anal. Chem. 2014, 86(8) 3708-3711. Bipolar electrochemistry (BPE) is nowadays well-known but relatively underexploited and still considered as...
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Site-Selective Synthesis of Janus-type Metal-Organic Framework Composites
Thursday, 06 March 2014
Yadnum S., Roche J., Lebraud E., Négrier P., Garrigue P., Bradshaw D., Warakulwit C., Limtrakul J., Kuhn A. Angew. Chem. Int. Ed. 2014, 126, 4082-4086. Herein, bipolar electrochemistry is applied in...
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Mapping the Electrogenerated Chemiluminescence Reactivity in Space: Mechanistic Insight into Model Systems Used in Immunoassays
Monday, 03 March 2014
Sentic, M., Milutinovic, M., Kanoufi, F., Manojlovic, D., Arbault S., Sojic, N.Chem. Sci. 2014, 5, 2568-2572. The remarkable characteristics of electrogenerated chemiluminescence (ECL) as a readout...
Enantioselective Recognition at Mesoporous Chiral Metal Surfaces
Wednesday, 19 February 2014
Wattanakit C., Bon Saint-Côme Y., Lapeyre V., Bopp P., Heim M., Yadnum S., Nokbin S., Warakulwit C., Limtrakul J., Kuhn A.Nature Comm. 2014, 5, article 3325. Chirality is widespread in natural...
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Straight-forward Synthesis of Ringed Particles
Thursday, 23 January 2014
Roche J., Loget G., Zigah D., Fattah Z., Goudeau B., Arbault S., Bouffier L., Kuhn A.Chem. Sci. 2014, 5, 1961-1966 Particles with sophisticated surface patterns have many potential applications...
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Impact of pNIPAM Microgel Size on their Ability to stabilize Pickering Emulsions
Wednesday, 22 January 2014
Destribats M., Lapeyre V., Sellier E., Varga I., Ravaine V., Schmitt V.Langmuir 2014, 30, 1768-1777. We study the influence of the particle size on the ability of poly(N-isoprolylacrylamide)...
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Asymmetrical Modification of Carbon Microfibers by Bipolar Electrochemistry in Acetonitrile
Friday, 10 January 2014
Ongaro M., Gambirasi A., Favaro M., Kuhn A., Ugo P.Electrochim. Acta 2014, 116, 421. This contribution presents the asymmetrical modification of carbon microfibers (CMF) with gold and polythiophene...
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Thiol-ene Clickable Hyaluronans: from Macro- to Nanogel
Wednesday, 25 December 2013
Hachet E., Sereni N., Pignot-Paintrand I., Ravaine V., Szarpak-Jankowska A., Auzély-Velty R.J. Coll. Interface Sci. 2014, 419, 52-55. The fabrication of hyaluronic acid (HA) nanogels using a...
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Enhanced Detection of Hydrogen Peroxide with Platinized Microelectrode Arrays for Analyses of Mitochondria Activities
Sunday, 01 December 2013
Ben-Amor S., Vanhove E., Sékli Belaïdi F., Charlot S., Colin D., Rigoulet M., Devin A., Sojic N., Launay J., Temple-Boyer P., Arbault S. Electrochimica Acta 2014, ASAP article Black...
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Electrochemical Transduction of DNA Hybridization at Modified Electrodes by using an Electroactive Pyridoacridone Intercalator
Thursday, 12 September 2013
Bouffier L., Wang B. S., Roget A., Livache T., Demeunynck M., Mailley P.Anal. Bioanal. Chem. 2014, 406(4), 1163-1172. A synthetic redox probe structurally related to natural pyridoacridones was...
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Optical Microwell Array for Large Scale Studies of Single Mitochondria Metabolic Responses
Sunday, 28 July 2013
Vajrala V. S., Suraniti E., Garrigue P., Goudeau B., Rigoulet M., Devin A., Sojic N., Arbault S.Anal. Bioanal. Chem. 2014, 406, 931-941. Forefront Paper Microsystems based on microwell arrays have...
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Lighting up Redox Propulsion with Luminol Electrogenerated Chemiluminescence
Monday, 08 July 2013
Bouffier L., Zigah D., Adam C., Sentic M., Fattah Z., Manojlovic D., Kuhn A., Sojic N.ChemElectroChem 2014, 1(1), 95-98. A blue-light-emitting swimmer driven by bipolar electrochemistry is reported...
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