Lefrançois P., Goudeau B., Arbault S.

Integrative Biology, 2018, in press

Phospholipid Giant Unilamellar Vesicles (GUVs) are usually prepared by electroformation in water, that is in a low-conductivity solution. We developed a protocol allowing their electroformation in the most common physiological buffer, the phosphate-buffered saline (PBS). This was achieved based on a specific sequence of increasing electrical fields and for the two usual electrode types for electroformation, namely Indium Tin Oxide-coated glass slides and Pt electrodes. These GUVs are stable over time (hour time-scale) and they can be isolated or micro-injected. The membrane composition was modified by adding cholesterol in order to adjust its mechanical properties. The optimal proportion of cholesterol vs. total phospholipid concentration was 20 mol % ratio which increases membrane rigidity and facilitates vesicle microinjection.

2018 09

TRPV1 activation induced by modulated or unmodulated 1800 MHz radiofrequency field exposure
lundi 18 juin 2018
Ruigrok, H.J., Arnaud-Cormos D., Hurtier A., Poque-Haro E., Poulletier de Gannes F., Ruffié G., Bonnaudin F, Lagroye I., Sojic N., Arbault S., Lévêque P., Veyret B. and Percherancier Y. Radiation Research, 2018, 189 (1), 95-103 The existence of effects of radiofrequency field exposure at environmental levels on living tissues and organisms remains controversial, in particular regarding... Read More...
Correlations between gaseous and liquid phase chemistries induced by cold atmospheric plasmas in a physiological buffer
lundi 18 juin 2018
Girard F., Peret M., Dumont N., Badets V., Blanc S., Gazeli K., Noël C., Belmonte T., Marlin L., Cambus J.P., Simon G., Sojic N., Held B., Clément F. and Arbault S. Phys. Chem. Chem. Phys., 2018, 20, 9198-9210 The understanding of plasma-liquid interactions is of major importance, not only in physical chemistry, chemical engineering and polymer science, but in biomedicine as well as to better... Read More...
Electroformation of Phospholipid Giant Unilamellar Vesicles in a Physiological Buffer
lundi 18 juin 2018
Lefrançois P., Goudeau B., Arbault S. Integrative Biology, 2018, in press Phospholipid Giant Unilamellar Vesicles (GUVs) are usually prepared by electroformation in water, that is in a low-conductivity solution. We developed a protocol allowing their electroformation in the most common physiological buffer, the phosphate-buffered saline (PBS). This was achieved based on a specific sequence of...
Des biopiles enzymatiques pour alimenter des circuits électroniques implantables
mardi 10 avril 2018
E. Suraniti, A.G. Mark, J. Roche, H. Richter, A. Kuhn, P. Fischer, N. Mano L’Actualité Chimique 2018, 427-428,127-128 Une micro-source d’énergie intégrée permettrait à des implants biomédicaux miniatures de fonctionner de façon autonome. Les piles ou batteries conventionnelles sont peu adaptées à cette utilisation car difficiles à miniaturiser. Des biopiles enzymatiques peuvent... Read More...
Current hot-spots and some more exotic topics
mardi 10 avril 2018
L. Bouffier, N. Sojic, A. Kuhn Current Opinion in Electrochemistry 2018, 7:A1–A4 This section of Current Opinion in Electrochemistry includes 17 articles contributed by groups from all over the world and we hope they will give the reader a representative vision of the latest achievements in the different areas of research. All selected authors are very active in frontier studies on physical and... Read More...
Une façon inhabituelle de briser la symétrie moléculaire: les métaux mesoporeux chiraux
jeudi 22 février 2018
A. Kuhn L’Actualité Chimique 2018, 426,19-24 L’analyse et la synthèse des composés chiraux est d’une importance cruciale dans de nombreux domaines de la société et de la science, y compris la médecine, la biologie, la chimie, la biotechnologie et l’agriculture. Ainsi, il existe un intérêt fondamental dans le développement de nouvelles approches pour la détection et la... Read More...
Gateway state-mediated, long-range tunnelling in molecular wires
jeudi 1 février 2018
S. Sangtarash,  A. Vezzoli,  H. Sadeghi, N. Ferri,  H. M. O'Brien,  I. Grace,  L. Bouffier, S. J. Higgins, R. J. Nichols, C. J. Lambert. Nanoscale 2018, DOI: 10.1039/C7NR07243K If the factors controlling the decay in single-molecule electrical conductance G with molecular length L could be understood and controlled, then this would be a significant step forward... Read More...
Tuning Electrochemiluminescence in Multi-Stimuli Responsive Hydrogel Films
lundi 8 janvier 2018
Haidong Li, Silvia Voci, Valérie Ravaine and Neso Sojic J. Phys. Chem. Lett., 2018, 9, 340–345 Luminescent and redox properties of stimuli-responsive hydrogel materials have been modulated by different external stimuli which trigger swelling or collapse of the polymer matrix. There is a very rapid development in the field of such “smart” materials particularly combined with other sensing... Read More...
Bipolar Conducting Polymer Crawlers Based on Triple Symmetry Breaking
vendredi 15 décembre 2017
B. Gupta, B. Goudeau, P. Garrigue, A. Kuhn Adv. Funct. Mater. 2018, 1705825 see also video clip in Zeste de Science Bipolar electrochemistry can be used in different ways to induce motion of an object, for example by generating gas bubbles in an asymmetric way or by a wireless self-regeneration mechanism due to the intrinsic symmetry breaking of this concept. Here a complementary approach is... Read More...