Electrochemistry of Biosystems
Linking Glucose Oxidation to Luminol-based Electrochemiluminescence using bipolar Electrochemistry
Asymmetric Modification of TiO2 Nanofibers with Gold by Electric-Field-Assisted Photochemistry
Stimuli-responsive microgels for electrochemiluminescence amplification

J. Kalecki, M. Cieplak, M. Dąbrowski, W. Lisowski, A. Kuhn,  P. Sindhu Sharma

ACS Sensors (2019) in press, doi.org/10.1021/acssensors.9b01878

Homogenous nanostructuration of molecularly imprinted polymer (MIP) films for follicle-stimulating hormone (FSH) sensing was achieved by using optimized colloidal crystals as a hard mold. Introduction of heating step after assembling colloidal crystals of silica beads promoted their adhesion.  Thus precise assembling of beads was not disturbed during further multistep of surface imprinting, and crack free hexagonal packing was maintained.  SEM imaging confirmed hexagonal packing of silica colloidal crystals as well as homogenous nanostructurization in MIP films. FSH immobilization over silica beads and later its derivatization with electroactive functional monomers was confirmed by XPS analysis. The nanostructured molecular recognition films prepared in this way were combined with an electrochemical transducer in order to design a capacitive impedimetry (CI) based chemosensing system. It was tested for the determination of FSH in the range from 0.1 fM to 100 pM in 10 mM MES buffer (pH = 4.2).  The detection limit of the chemosensor was 0.1 fM showing a high selectivity with respect to common protein interferences as well as other protein hormones of the gonadotropin family.

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Thumbnail J. Kalecki, M. Cieplak, M. Dąbrowski, W. Lisowski, A. Kuhn,  P. Sindhu Sharma ACS Sensors (2019) in press, doi.org/10.1021/acssensors.9b01878 Homogenous nanostructuration of molecularly imprinted...
Thumbnail S. Assavapanumat,  M. Ketkaew,  A. Kuhn, C.Wattanakit J. Am.Chem.Soc. 141 (2019) 18870(Front Cover) see also CNRS press release The enantioselective synthesis of chiral compounds is of crucial...
Thumbnail C. Wattanakit, A. Kuhn Chemistry-A European Journal (2019) in press The concept of encoding molecular information in bulk metals has been proposed over the past decade. The structure of various...