Dezest M., Chavatte L., Bourdens M., Quinton D., Camus M., Garrigues L., Descargues P., Arbault S., Burlet-Schiltz O., Casteilla L., Clément F., Planat V., Bulteau A.L.

Scientific Reports, 2017, 7, 41163

Compelling evidence suggests that Cold Atmospheric Pressure Plasma (CAPP) has potential as a new cancer therapy. However, knowledge about cellular signaling events and toxicity subsequent to plasma treatment is still poorly documented. The aim of this study was to focus on the interaction between 3 different types of plasma (He, He-O2, He-N2) and human epithelial cell lines to gain better insight into plasma-cell interaction. We provide evidence that reactive oxygen and nitrogen species (RONS) are inducing cell death by apoptosis and that the proteasome, a major intracellular proteolytic system which is important for tumor cell growth and survival, is a target of (He or He-N2) CAPP. However, RONS are not the only actors involved in cell death; electric field and charged particles could play a significant role especially for He-O2 CAPP. By differential label-free quantitative proteomic analysis we found that CAPP triggers antioxidant and cellular defense but is also affecting extracellular matrix in keratinocytes. Moreover, we found that malignant cells are more resistant to CAPP treatment than normal cells. Taken together, our findings provide insight into potential mechanisms of CAPP-induced proteasome inactivation and the cellular consequences of these events.

2017 33

Thumbnail Dezest M., Bulteau A.L., Quinton D., Chavatte L., Le Béchec M., Arbault S., Salvayre A.N., Cousty S., Clément F. PLOS One, 2017, 12(3) Cold atmospheric pressure plasmas (CAPPs) are known to have...
Thumbnail C. Wattanakit, T. Yutthalekha, S. Asssavapanumat, V. Lapeyre, A. Kuhn Nature Comm. 2017, 8, 2087 see also CNRS press release Asymmetric synthesis of molecules is of crucial importance to obtain...
Thumbnail Giovanni Valenti, Sabina Scarabino, Bertrand Goudeau, Andreas Lesch, Milica Jovic, Elena Villani, Milica Sentic, Stefania Rapino, Stephane Arbault, Francesco Paolucci, and Neso Sojic J. Am. Chem....
Thumbnail V. Eßmann, S. Voci, G. Loget, N. Sojic, W. Schuhmann, A. Kuhn J. Phys. Chem. Lett. 2017,8, 4930–4934   Bipolar electrochemistry has been shown to enable and control various kinds of propulsion of...
Thumbnail Guangnan Meng, Vinothan N. Manoharan and Adeline Perro Soft Matter, 2017,13, 6293-6296 We design polystyrene–poly(N′-isopropylacrylamide-co-acrylic acid) core–shell particles that exhibit...
Thumbnail J. Gao, S. Chen, F. AlTal, S. Hu, L. Bouffier, G. Wantz.  ACS Appl. Mater. Interfaces, 2017, 9, 32405–32410.  A linear array of aluminum discs is deposited between the driving electrodes of an...
Thumbnail B. Gupta, B. Goudeau, A. Kuhn Angew.Chem. Int. Ed. 2017, 56, 14183-14186 see also Highlight in Chemistry Views and CNRS press release Electrochemical actuation of conducting polymers usually...
Thumbnail Z. Ali Fattah, L. Bouffier, A. Kuhn Applied Materials Today 2017, 9, 259–265, COVER Self-propelled micro/nanoswimmers performing directional motion are of great potential nowadays in the frame of...
Thumbnail T.D. Le, D. Lasseux, X.P. Nguyen, G. Vignoles, N. Mano, A. Kuhn Chemical Engineering Science 2017, 173, 153–167 A multi-scale model of diffusion/reaction at play in a porous electrode is developed...
Thumbnail M. Dabrowski, M. Cieplak, K. Noworyta, M. Heim, W. Adamkiewicz, A. Kuhn, P. S. Sharma, W. Kutner J. Mater. Chem. B, 2017,5, 6292-6299 Molecular imprinting in polymers leads, among others, to...
Thumbnail L. Bouffier, T. Doneux.  Current Opinion in Electrochemistry, DOI: 10.1016/j.coelec.2017.06.015. Electrochemistry and fluorescence microscopy are two complementary transduction techniques that offer synergetic...
Thumbnail G. Di Martino,V. A. Turek,  A. Lombardi, I.  Szabó, B. de Nijs,  A. Kuhn, E. Rosta, J.J. Baumberg Nano Lett. 2017, 17, 4840-4845 We study in real time the optical response of individual plasmonic...
Thumbnail Rémy Bois, Sabina Scarabino, Valérie Ravaine, Neso Sojic Langmuir, 2017, DOI: 10.1021/acs.langmuir.7b01585 We describe a method to confine electrochemiluminescence (ECL) at the oil-water interface of emulsion...
Thumbnail D. Zigah, O. Fontaine World Scientific, 2017, ISBN: 978-1-78634-353-6   Bioelectrochemical Systems (BESs) are innovative and sustainable devices. They combine biological and electrochemical...
Thumbnail C. Bodin,  E. Mourad,  D. Zigah,  S. Le Vot,  S. A Freunberger,  F. Favier, O. Fontaine Faraday Discuss., 2017,DOI: 10.1039/C7FD00174F   Nowadays, commercial supercapacitors are based on purely...
Thumbnail S. Tiewcharoen, C. Warakulwit, V. Lapeyre, P. Garrigue, L. Fourier, C. Elissalde, S. Buffière, P. Legros, M. Gayot, J. Limtrakul, A. Kuhn Angew.Chem.Int.Ed. (2017) 56, 11431-11435, COVER, HOT...
Thumbnail G. Di Martino, V. A. Turek,  C. Tserkezis, A. Lombardi, A. Kuhn, J.J. Baumberg Faraday Discuss., 2017, in press,doi 10.1039/C7FD00130D We study the optical response of individual nm-wide plasmonic...
Thumbnail A. G. Mark, E. Suraniti, J. Roche, H. Richter, A. Kuhn, N. Mano, P. Fischer Lab Chip, 2017, 17, 1761 HOT PAPER see also CNRS press release A variety of diagnostic and therapeutic medical...
Thumbnail I. Malytska, C. Mézière, M. Keilar, L. Hirsch, G. Wantz, N. Avarvari, A. Kuhn, L. Bouffier. Journal of Physical Chemistry C 2017, 121, 12921-12927.   Bipolar electrochemistry has recently emerged as a very...
Thumbnail L. Bouffier, S. Reculusa, V. Ravaine, A. Kuhn. ChemPhysChem 2017, 18, 2637-2642.   A new simple and versatile method for the preparation of surface wetting gradients is proposed. It is based on the...
Thumbnail C.A. Busatto, H. Labie, V. Lapeyre, R. Auzely-Velty, A. Perro, N. Casis, J. Luna, D.A. Estenoz, V. Ravaine Journal of Colloid and Interface Science, 493, 2017, 356–364. Polymer microgels have received...
Thumbnail L. Bouffier, N. Sojic, A. Kuhn Electrophoresis, 2017, 38, 2687-2694. A bipolar electrode is quite unconventional as it behaves simultaneously as an anode and a cathode in contrast to a classic...
Thumbnail M. Dabrowski, M. Cieplaka, P. Sindhu Sharma, P. Borowicz, K. Noworyta, W. Lisowski, F. D'Souza, A. Kuhn, W. Kutner Biosens.&Bioelectron.2017, 94, 155-161 Nanostructured artificial receptor...
Thumbnail Nenad R. Filipovic, Hana Elshaflu, Sonja Grubic, Ljiljana S. Jovanovic, Marko Rodic, Irena Novakovic, Aleksandar Malecevic, Ivana S. Djordjevic, Haidong Li, Neso Sojic, Aleksandar Marinkovic,...
Thumbnail Haidong Li, Silvia Voci, Antoine Wallabregue, Catherine Adam, Geraldine M. Labrador, Romain Duwald, Irene Hernández Delgado, Simon Pascal, Johann Bosson, Jérôme Lacour, Laurent BouffierNeso...