Bouffier L., Yiu H. H. P., Rosseinsky M. J.
Langmuir 2011, 27, 6185-6192.

Spherical magnetite nanoparticles (MNPs, 24 nm in diameter) were sequentially functionalized with trimethoxysilylpropyldiethylenetriamine (TMSPDT) and a synthetic DNA intercalator, namely, 9-chloro-4H-pyrido[4,3,2-kl]acridin-4-one (PyAcr), in order to promote DNA interaction. The designed synthetic pathway allowed control of the chemical grafting efficiency to access MNPs either partially or fully functionalized with the intercalator moiety. The newly prepared nanomaterials were characterized by a range of physicochemical techniques: FTIR, TEM, PXRD, and TGA. The data were consistent with a full surface coverage by immobilized silylpropyldiethylenetriamine (SPDT) molecules, which corresponds to 22300 SPDT molecules per MNP and a subsequent (4740 − 2940) PyAcr after the chemical grafting step (i.e., 2.4 PyAcr/nm2). A greater amount of PyAcr (30600) was immobilized by the alternative strategy of binding a fully prefunctionalized shell to the MNPs with up to 16.1 PyAcr/nm2. We found that the extent of PyAcr functionalization strongly affects the resulting properties and, particularly, the colloidal stability as well as the surface charge estimated by ζ-potential measurement. The intercalator grafting generates a negative charge contribution which counterbalances the positive charge of the single SPDT shell. The DNA binding capability was measured by titration assay and increases from 15 to 21.5 μg of DNA per mg of MNPs after PyAcr grafting (14−20% yield) but then drops to only 2 μg for the fully functionalized MNPs. This highlights that even if the size of the MNPs is obviously a determining factor to promote surface DNA interaction, it is not the only limiting parameter, as the mode of binding and the interfacial charge density are essential to improve loading capability.

 

 

Lenz J., Trieu V., Hempelmann R., Kuhn A.
Electroanalysis 2011, 23, 1186-1192.

We report two methods to prepare ordered macroporous ruthenium oxide electrodes based on a template approach and their possible applications as potentiometric and amperometric sensors. The very stable porous electrodes have been prepared using two techniques to assemble the colloidal template: the controlled evaporation (CE) technique and the Langmuir-Blodgett (LB) technique, followed by electrochemical deposition of ruthenium oxide. The increased surface area of the macroporous structure makes them attractive for electroanalytical applications such as potentiometric and amperometric sensors as is illustrated for pH sensing and direct oxidation of NADH, which is an ingredient in dehydrogenase based biosensors.

2011-10

Mertins O., Messina P., Labbé E., Arbault S., Lemaitre F., Buriez O., Amatore C.
Inorganica Chimica Acta 2011, 374(1), 59-68

Transport, reactivity and interactions of anti-tumor ferrocene-based compounds within lipid environments have been investigated electrochemically at a glassy carbon electrode (GCE) modified with a lipid bilayer film. Cyclic voltammetry performed in the presence of an adsorbed bilipid film in a H2O/EtOH (8/2) mixture, clearly showed that the affinity of the starting neutral ferrocifen derivatives towards the lipidic bilayer depends on both their size and their polarity. Likewise, the electrogenerated ferrocenium derivatives were expulsed reversibly from the bilayer in agreement with their positive charge. The reactivity of ferrocifen compounds was also investigated under the same conditions and in the presence of a base in order to trigger an intramolecular ferrocene-mediated (−2e −2H+) process within the lipidic film. Under these conditions, two distinct sequential electrochemical behaviors were observed depending on the base availability in the lipid layer, the magnitude of the corresponding two electrons wave being limited by the base concentration in the film. A full two-electron oxidation process, was observed as long as the amount of base present into the lipid layer was high enough to titrate the two protons released in the two-electron process. When this was sub-stoichiometric a second wave was observed at higher potential values representing the one electron oxidation of the ferrocifen excess.

2011-13

Loget G., Lapeyre V., Garrigue P., Warakulwit C., Limtrakul J., Delville M.-H., Kuhn A.
Chem.Mater. 2011, 23, 2595-2599

A bulk procedure based on bipolar electrochemistry is proposed for the generation of Janus-type carbon tubes. The concept is illustrated with carbon tubes, that are selectively modified at their ends with various metals and conducting polymers. No surface or interface is required to break the symmetry and therefore this approach could be used for the mass production of Janus micro- and nanoobjects. We show evidence that the technique is very versatile, allowing to choose the kind of material that is deposited and whether the end product is mono- or bifunctionalized.

2011-12

Meunier A., Jouannot O., Fulcrand R., Fanget I., Bretou M., Karatekin E., Arbault S., Guille M., Darchen F., Lemaitre F., Amatore C.
Angew. Chem. Int. Ed. 2011, 50(22), 5081-5084

A microdevice based on transparent indium tin oxide (ITO) electrodes allows simultaneous total internal reflection fluorescence microscopy and amperometric measurements. Use of the device in the coupled optical and electrochemical detection of single exocytotic events is demonstrated with enterochromaffin BON cells.

2011-14

Thumbnail S. Assavapanumat, T. Yutthalekha, P. Garrigue, B. Goudeau, V. Lapeyre, A. Perro, N. Sojic, C. Wattanakit, A. Kuhn Angew. Chem. Int. Ed. 58 (2019) 3471 (Back Cover) see also CNRS press release...
Thumbnail S. Voci, B. Goudeau, G. Valenti, A. Lesch, M. Jović, S. Rapino, F. Paolucci, S. Arbault, N. Sojic J. Am. Chem. Soc., 140,2018, 14753-14760 Herein is reported a surface-confined microscopy based...
Thumbnail Hanan Al-Kutubi, Silvia Voci, Liza Rassaei, Neso Sojic, Klaus Mathwig Chem .Sci., 9, 2018, (2018 Chemical Science HOT Article Collection) Microfabricated nanofluidic electrochemical devices offer a...
Thumbnail L. Bouffier, S. Arbault, A. Kuhn, N. Sojic Techniques de l’Ingénieur. 2018. P156 Les mesures sélectives et sensibles dans des échantillons complexes tels que l’urine ou le sang sont devenues des outils...
Thumbnail H. Labie, A. Perro, V. Lapeyre, B. Goudeau, B. Catargi, R. Auzély, V. Ravaine Journal of Colloid and Interface Science 535 (2019) 16–27 A simple route to deliver on demand hydrosoluble molecules such...
Thumbnail E.Krisch, B. Gyarmati, D. Barczikai, V. Lapeyre, B.A. Szilágyi, V. Ravaine, A. Szilágyi European Polymer Journal 105 (2018) 459–468 Redox and pH responsive hydrogels were synthesized, which can...
Thumbnail L. Zhang, B. Gupta, B. Goudeau, N. Mano, A. Kuhn J. Am. Chem. Soc 140 (2018) 15501 Collecting electrochemical information concerning the presence of molecules in a solution is usually achieved by measuring...
Thumbnail L. Adam, A. Kuhn Techniques de l'Ingénieur (2019)Ref:IN189 V1 Les molécules chirales sont d’un grand intérêt dans un certain nombre de domaines tels que la médecine, la biologie ou encore la chimie....
Thumbnail J.-P. Douliez, A. Perro, J.-P. Chapel, B. Goudeau and L. Béven Small 2018, 1803042 A template-free all-aqueous bulk preparation of robust hollow capsules having a gelatin shell from all-in-water...
Thumbnail G. Salinas, B. A. Frontana-Uribe, S. Reculusa, P. Garrigue, A. Kuhn Anal.Chem. 90 (2018) 11770 Highly ordered macroporous electrodes of the conducting polymer poly-3,4-ortho-xylendioxythiophene...
Thumbnail T. D. Le, L. Zhang, S. Reculusa, G.Vignoles, N. Mano, A. Kuhn, D. Lasseux ChemElectroChem 6 (2018) 173 This article reports on a procedure to predict the optimal thickness of cylindrical porous...
Thumbnail A. de Poulpiquet, B. Goudeau, P. Garrigue, N. Sojic, S. Arbault, T. Doneux, L. Bouffier. Chem. Sci. 2018, 9, 6622-6628. The coupling between electrochemistry and fluorescence confocal laser scanning...
Thumbnail V. Pirenne, G. Kurtay, S. Voci, L. Bouffier, N. Sojic, F. Robert, D. M. Bassani, Y. Landais. Org. Lett. 2018, 20 (15), 4521-4525. Eosin-Y (EY)-mediated alkylsulfonyl cyanation of olefins was shown...
Thumbnail I. Hernández Delgado, S. Pascal, C. Besnard, S. Voci, L. Bouffier, N. Sojic, J. Lacour. Chem. Eur. J. 2018, 24 (40), 10186-10195. A series of nine C‐functionalized cationic diazaoxa triangulene...
Thumbnail E. Suraniti, P. Merzeau, J. Roche, S. Gounel, A. G. Mark, P. Fischer, N. Mano, A. Kuhn Nature Comm. 2018, 9:3229 see also CNRS press release Chemical systems do not allow the coupling of energy from...
Thumbnail D. Kos, H. P. A. G. Astier, G. D. Martino, J. Mertens, H. Ohadi, D. De. Fazio, D. Yoon, Z. Zhao, A. Kuhn, A. C. Ferrari, C. J. B. Ford, J. J. Baumberg Small 14 (2018) 1801599 Nanoactuators are a key...
Thumbnail Ruigrok, H.J., Arnaud-Cormos D., Hurtier A., Poque-Haro E., Poulletier de Gannes F., Ruffié G., Bonnaudin F, Lagroye I., Sojic N., Arbault S., Lévêque P., Veyret B. and Percherancier Y. Radiation...
Thumbnail Girard F., Peret M., Dumont N., Badets V., Blanc S., Gazeli K., Noël C., Belmonte T., Marlin L., Cambus J.P., Simon G., Sojic N., Held B., Clément F. and Arbault S. Phys. Chem. Chem. Phys., 2018, 20,...
Thumbnail Lefrançois P., Goudeau B., Arbault S. Integrative Biology, 2018, in press Phospholipid Giant Unilamellar Vesicles (GUVs) are usually prepared by electroformation in water, that is in a...
Thumbnail E. Suraniti, A.G. Mark, J. Roche, H. Richter, A. Kuhn, P. Fischer, N. Mano L’Actualité Chimique 2018, 427-428,127-128 Une micro-source d’énergie intégrée permettrait à des implants biomédicaux...