M. Dąbrowski, A. Ziminska, J. Kalecki, M. Cieplak, W. Lisowski, R. Maksym, S. Shao, F. D’Souza, A. Kuhn, P. S. Sharma

ACS Applied Materials and Interfaces 11 (2019) 9265

We present an improved approach for the preparation of highly selective and homogeneous molecular cavities in molecularly imprinted polymers (MIP) via the combination of surface imprinting and semi-covalent imprinting. Towards that, first a colloidal crystal mold was prepared via the Langmuir-Blodgett technique. Then, human chorionic gonadotropin (hCG) template protein was immobilized on the colloidal crystal mold. Later, hCG derivatization with electroactive functional monomers via amide chemistry was performed.  In a final step, optimized potentiostatic polymerization of 2,3′-bithiophene enabled depositing an MIP film as macroporous structure. This synergistic strategy resulted in the formation of molecular imprinted cavities exclusively on the internal surface of the macropores, which were accessible after dissolution of silica molds. The recognition of hCG by the macroporous MIP film was transduced with the help of electric transducers, namely extended-gate field-effect transistors (EG-FET) and capacitive impedimetry (CI). These readout strategies offer the ability to create chemosensors for the label-free determination of hCG hormone. Other than the simple confirmation of pregnancy, hCG assay is a common tool for the diagnosis and follow-up of ectopic pregnancy or trophoblast tumors. Concentration measurements with these EG-FET and capacitive impedimetry based devices allowed real-time measurements of hCG in the range of 0.8 to 900 fM and 0.17 to 2.0 fM, respectively in 10 mM carbonate buffer (pH = 10). Moreover, the selectivity of the chemosensors with respect to protein interferences was very high. 

2019 02

Thumbnail J. Kalecki, M. Cieplak, M. Dąbrowski, W. Lisowski, A. Kuhn,  P. Sindhu Sharma ACS Sensors (2019) in press, doi.org/10.1021/acssensors.9b01878 Homogenous nanostructuration of molecularly imprinted...
Thumbnail C. Wattanakit, A. Kuhn Chemistry-A European Journal (2020) 26, 2993-3003 The concept of encoding molecular information in bulk metals has been proposed over the past decade. The structure of various types of...
Thumbnail S. Assavapanumat,  M. Ketkaew,  A. Kuhn, C.Wattanakit J. Am.Chem.Soc. 141 (2019) 18870(Front Cover) see also CNRS press release The enantioselective synthesis of chiral compounds is of crucial...
Thumbnail A. Bouchet-Spinelli, E. Descamps, J. Liu, A. Ismail, P. Pham, F. Chatelain, T. Leïchlé, L. Leroy, P. N. Marche, C. Raillon, A. Roget, Y. Roupioz, N. Sojic, A. Buhot, V. Haguet, T. Livache and P....
Thumbnail T.D. Le, L. Zhang, A. Kuhn, N. Mano, G. Vignoles, D. Lasseux J.Electroanal.Chem. (2019) in press Multiscale modelling of coupled diffusion and serial reduction reactions in porous micro-electrodes is...
Thumbnail S. Assavapanumat, M. Ketkaew, P. Garrigue, V Lapeyre, S Reculusa, C Wattanakit, A Kuhn ChemCatChem 11 (2019) 5951(Front Cover) Cover Profile Electrochemical water splitting is a crucially...
Thumbnail S. Assavapanumat, B. Gupta, G. Salinas, B. Goudeau, C. Wattanakit, A. Kuhn ChemComm 55 (2019) 10956 We report the synthesis of a hybrid bilayer, being composed of a free-standing conducting polymer...
Thumbnail A. Kuhn ChemPhysChem (2019) in press, DOI: 10.1002/cphc.201900932  Physical Chemistry is positioned at the interface of chemistry and physics and can be considered as a key player in modern science....
Thumbnail A. Dauphin, A. Akchach, S. Voci, A. Kuhn, G. Xu, L. BouffierN. Sojic J. Phys. Chem. Lett. 2019, 10, 318-5324 There is a very rapid development of original systems that can be remotely...
Thumbnail Y. Zhao, J. Yu, G. Xu, N. Sojic, G. Loget J. Am. Chem. Soc. 2019, 141, 13013−13016 We introduce the photo-induced electrochemiluminescence (P-ECL) of the model ECL system involving the...
Thumbnail L. Zhang, C. Carucci, S. Reculusa, B. Goudeau, P. Lefrançois, S. Gounel, N. Mano, A. Kuhn Chem Electro Chem2019,6, 4980-4984 (Cover). The immobilization of bilirubin oxidase (BOD) on macroporous gold...
Thumbnail A. Perro, L. Giraud, N. Coudon, S. Shanmugathasan, B. Goudeau, J.-P. Douliez and V. Ravaine Journal of Colloid and Interface Science2019 548, 275-283. Coacervation is a phase separation process...
Thumbnail M.-C. Tatry, E. Laurichesse, A. Perro, V. Ravaine and V. Schmitt Journal of Colloid and Interface Science2019, 548, 1-11. The aim of the paper is to examine the adsorption kinetics of soft...
Thumbnail S. Ramalingam, G. Le Bourdon, E. Pouget, A. Scalabre, J. Raghava Rao and A. Perro J. Phys. Chem B2019, 123 (7), 1708-1717. The design of nanocarriers containing hydrophobic and hydrophilic...
Thumbnail H. Labie, A. Perro, V. Lapeyre, B. Goudeau, B. Catargi, R. Auzély, V. Ravaine  Journal of Colloid and Interface Science 2019, 535, 16-27. A simple route to deliver on demand hydrosoluble molecules...
Thumbnail K. Vindas, L. Leroy, P. Garrigue, S. Voci, T. Livache, S. Arbault, N. Sojic, A. Buhot, E. Engel Anal. Bioanal. Chem., 2019. Remote detection by surface plasmon resonance (SPR) is demonstrated...
Thumbnail F. Zinna, S. Voci, L. Arrico, E. Brun, A. Homberg, L. Bouffier, T. Funaioli, J. Lacour, N. Sojic, L. Di Bari Angew. Chem. Int. Ed.,2019. The first observation of circular polarization of...
Thumbnail K. Mathwig, N. Sojic Journal of Analysis and Testing,2019, accepted. In ion-annihilation electrochemiluminescence (ECL), luminophore ions are generated by oxidation as well as reduction at electrodes...
Thumbnail J. Zhang, S. Arbault, N. Sojic, D. Jiang Annu. Rev. Anal. Chem. 2019. 12. Electrochemiluminescence (ECL) is a widely used analytical technique with the advantages of high sensitivity and low...
Thumbnail B. Gupta, M. C. Afonso, L. Zhang, C. Ayela, P. Garrigue, B. Goudeau, A. Kuhn ChemPhysChem 7 (2019) 941 (Cover) Combining the actuation of conducting polymers with additional functionalities is an...
Thumbnail M. Dąbrowski, A. Ziminska, J. Kalecki, M. Cieplak, W. Lisowski, R. Maksym, S. Shao, F. D’Souza, A. Kuhn, P. S. Sharma ACS Applied Materials and Interfaces 11 (2019) 9265 We present an improved...
Thumbnail I. Malytska, Th. Doneux, M. Bougouma, A. Kuhn, L. Bouffier. J. Phys. Chem. C, 2019, in press Doping of a transition-metal dichalcogenide deposited onto a conducting surface acting as bipolar...