E. Pedraza, A. Karajić, M. Raoux, R. Perrier, A. Pirog, F. Lebreton, S. Arbault, J. Gaitan, S. Renaud, A. Kuhn, J. Lang

Lab Chip, 2015, 15, 3880–3890

We are developing a cell-based bioelectronic glucose sensor that exploits the multi-parametric sensing
ability of pancreatic islet cells for the treatment of diabetes. These cells sense changes in the concentration
of glucose and physiological hormones and immediately react by generating electrical signals. In our sensor,
signals from multiple cells are recorded as field potentials by a micro-electrode array (MEA). Thus, cell
response to various factors can be assessed rapidly and with high throughput. However, signal quality and
consequently overall sensor performance rely critically on close cell–electrode proximity. Therefore, we
present here a non-invasive method of further exploiting the electrical properties of these cells to guide
them towards multiple micro-electrodes via electrophoresis. Parameters were optimized by measuring the
cell's zeta potential and modeling the electric field distribution. Clonal and primary mouse or human
β-cells migrated directly to target electrodes during the application of a 1 V potential between MEA
electrodes for 3 minutes. The morphology, insulin secretion, and electrophysiological characteristics were
not altered compared to controls. Thus, cell manipulation on standard MEAs was achieved without introducing
any external components and while maintaining the performance of the biosensor. Since the analysis
of the cells' electrical activity was performed in real time via on-chip recording and processing, this work
demonstrates that our biosensor is operational from the first step of electrically guiding cells to the final
step of automatic recognition. Our favorable results with pancreatic islets, which are highly sensitive and
fragile cells, are encouraging for the extension of this technique to other cell types and microarray devices.

2015-13

Thumbnail N. Sojic, S. Arbault, L. Bouffier, A. Kuhn. Book Chapter in Luminescence in Electrochemistry Eds. F. Miomandre, P. Audebert. 2017. Pp. 257-291. Springer The great success of electrogenerated...
Thumbnail L. Bouffier, S. Arbault, A. Kuhn, N. Sojic Techniques de l’Ingénieur. 2018. P156 Les mesures sélectives et sensibles dans des échantillons complexes tels que l’urine ou le sang sont devenues des...
Thumbnail N. Sojic BOOK Royal Society of Chemistry Electrogenerated chemiluminescence (ECL) is a powerful and versatile analytical technique, which is widely applied for biosensing and successfully...
Thumbnail Arbault S.In Chemical Sensors and Biosensors, Eds. R. Lalauze and N. Jaffrezic-Renault, ISTE-Wiley, London, 2012. Book Chapter Technological needs for chemical, ionic and biological species detection...
Fibre-optic Biosensors
21 January 2013
Thumbnail Sojic N.,In Chemical Sensors and Biosensors, Eds. R. Lalauze and N. Jaffrezic-Renault, ISTE-Wiley, London, 2012. Book Chapter Technological needs for chemical, ionic and biological species...
Thumbnail Sojic N., Kuhn A.,Techniques de l’Ingénieur, Référence P150, 2012. Les capteurs sont devenus indispensables pour de nombreux aspects de notre vie, avec des exemples d’application allant de la...
Thumbnail Heim M., Kuhn A.,In Handbook of Electrochemistry, Springer, in press, 2013. Book Chapter In recent years, the field of highly ordered macroporous thin films coated onto solid electrode surfaces...