Les mesures sélectives et sensibles dans des échantillons complexes tels que l’urine ou le sang sont devenues des outils indispensables dans le domaine du diagnostic. Le développement de nouvelles méthodes d’analyse, plus sensibles, dynamiques, à haut débit représente un défi d’une grande importance sociétale. L’électrochimiluminescence - ou ECL - constitue une méthode largement commercialisée pour l’immunodosage. Cet article présente des travaux qui portent, d’une part, sur la compréhension fine des mécanismes de l’ECL mis en jeu et, d’autre part, sur le développement de l’ECL pour la bioanalyse, avec un accent particulier sur le couplage entre l’électrochimie bipolaire et l’ECL.
C. Wattanakit, A. Kuhn
The development of chiral electrodes plays an important role in various areas, including chemical science, materials engineering, analytical chemistry, pharmaceutics, cosmetics, and catalysis. Over the past decades, various approaches have been used to design chiral electrodes, such as the adsorption of chiral/achiral molecules on metal electrode surfaces, binding of chiral ligands to metal surfaces, cutting a bulk metal to break the symmetric metal structure, and molecular imprinting. In this chapter, we discuss recent progress related to the development of such chiral metal electrodes by following these different strategies. In addition, their potential applications with respect to enantioselective analysis, asymmetric synthesis, and chiral separation will be discussed. This chapter will also illustrate perspectives for the fabrication and use of chiral metal electrodes in the frame of chiral technologies.
N. Sojic, S. Arbault, L. Bouffier, A. Kuhn.
Book Chapter in Luminescence in Electrochemistry
Eds. F. Miomandre, P. Audebert. 2017. Pp. 257-291. Springer
The great success of electrogenerated chemiluminescence (ECL) in analytical chemistry can be measured by the widespread use of the technology in different fields, ranging from basic research to commercial clinical and biological applications. Indeed, this remarkable readout method offers intrinsic advantages by comparison with other transduction methods: high sensitivity, extremely wide dynamic range, and insensitivity to matrix effects. In addition, its versatility allows exploiting various types of biomolecular interactions and therefore to detect specifically targeted analytes of biological interest such as proteins, nucleic acids, and enzymatic substrates. Numerous assay formats, biosensors, or analytical strategies with new ECL labels or with label-free approaches have been proposed by using nanostructured materials: carbon nanotubes, metal or doped nanoparticles, graphene, carbon dots, quantum dots, or ultrathin films. The development of analytical ECL has also been fueled by discovering novel luminophores and efficient co-reactants and also by deciphering the complexity of the ECL mechanisms at the minute scale. The combination of ECL with microfluidics, paper-based materials, bipolar electrochemistry, and portable miniaturized devices has led to various intriguing and promising analytical applications.
Electrogenerated chemiluminescence (ECL) is a powerful and versatile analytical technique, which is widely applied for biosensing and successfully commercialized in the healthcare diagnostic market. After introducing the fundamental concepts, this book will highlight the recent analytical applications with a special focus on immunoassays, genotoxicity, imaging, DNA and enzymatic assays. The topic is clearly at the frontier between several scientific domains involving analytical chemistry, electrochemistry, photochemistry, materials science, nanoscience and biology. This book is ideal for graduate students, academics and researchers in industry looking for a comprehensive guide to the different aspects of electrogenerated chemiluminescence.
Arbault S.
In Chemical Sensors and Biosensors, Eds. R. Lalauze and N. Jaffrezic-Renault, ISTE-Wiley, London, 2012. Book Chapter
Technological needs for chemical, ionic and biological species detection are giving rise to continuous research and development in physico-chemistry and biology. The constant progress being made in the theoretical and technological aspects concerning studies and developments of chemical sensors, biosensors and biochips is presented in this book by different scientists and professors from different universities and constitutes an updating of the state of the art for chemical sensors, biosensors and biochips.
This book places a large emphasis on interaction between chemical and biological species, in a gaseous or liquid state, and details mineral and biological materials acting as sensitive elements. The role of electrical, electrochemical, piezoelectric and optical transducers in detection mechanisms are presented through their developments and from a performance point-of-view. Micro-reactors, nanotechnologies and flexible substrates, are considered in relation to their role in neural networks.
