G. Salinas, M. Niamlaem, A. Kuhn, S. Arnaboldi
Current Opinion in Colloid & Interface Science, 61 (2022) 101626
Chirality governs many chemical and biological processes, since most active substances are chiral. Enantiomers are mirror images of the same molecule that can exhibit significant differences in terms of biochemical activity, toxicity and transport mechanisms in the human body. Thus, developing effective methods to achieve chiral recognition is of great significance and has been a hot topic in chemo/biological studies. In recent years, electrochemical methods have received considerable attention in this field since they can offer several advantages over chromatographic or spectroscopic methods. In this review, a comprehensive and critical description of the trends in electrochemical chiral recognition over the last five years is presented. In addition, some unconventional electrochemical methods for the transduction of chirality at different length scales are described and discussed.
S. Knezevic, L. Bouffier, B. Liu, D. Jiang, N. Sojic
Current Opinion in Electrochemistry, in press
Electrochemiluminescence (ECL) is a light-emitting phenomenon triggered by electrochemistry. The resulting optical signal enables spatiotemporal resolution of the luminescent signal that can be imaged with high accuracy. The field of ECL is evolving rapidly from an analytical method widely applied in biosensing to a novel microscopy technique. The efficient generation of ECL depends on the electrode materials, the luminophore/coreactant systems, and experimental configurations, focusing research on improving these parameters. Nowadays, ECL enables the analysis of transient events and micro/nanometric objects, surface boundaries, interfaces and physical phenomena, as well as catalytic and biological processes. Since ECL microscopy offers many novel applications, this article will describe first the context and then the most recent advances in ECL imaging of single objects and living cells or subcellular structures.
R. Mhanna, N. Durand, P. Savel, H. Akdas-Kiliç, S. Abdallah, D.-L. Versace, O. Soppera, J.-L. Fillaut, N. Sojic, J.-P. Malval
Chem. Comm., 2022, 58, 9678-9681
We present an original stereolithography strategy based on multibranched Ru-complexes with a high two-photon initiating ability allowing the ‘one-pot’ direct laser writing of ECL-active materials deposited onto electro-active surfaces at µm scale.
D. Han, B. Goudeau, V. Lapeyre, V. Ravaine, D. Jiang, D. Fang and N. Sojic
Biosens. Bioelectron., 2022, 216, 114640
Bead-based assays are successfully combined with electrochemiluminescence (ECL) technology for detection of a wide range of biomarkers. Herein, we demonstrate a novel approach to enhance the ECL signal by decorating micrometric beads with [Ru(bpy)3]2+-grafted microgels (diameter ∼100 nm). Rapid and stable light emission was spatially resolved at the level of single functionalized beads. An enhancement of the ECL signal of microgel-labeled beads by 9-fold was observed in comparison to molecularly linked [Ru(bpy)3]2+ beads prepared by a sandwich immunoassay or an amide bond. Imaging the ECL signal at the single bead level shows that the size of the ECL-emitting layer is extended using the microgels. The reported method offers a great promise for the optimization of bead-based ECL detection and subsequent development of ECL microscopy.
S. Butcha, J. Yu, Z. Pasom, B. Goudeau, C. Wattanakit, N. Sojic, A. Kuhn
ChemComm 58 (2022) 10707-10710
Chiral-imprinted mesoporous Pt-Ir alloy surfaces were combined in a synergetic way with electrochemiluminescence (ECL) to detect the two enantiomers of phenylalanine (PA) as a model compound, acting simultaneously as a chiral target and as a co-reactant to generate significant differences in ECL signals. The chiral features of the metal surfaces are converted into an enantioselective electrogeneration of the excited state of the [Ru(bpy)3]2+ dye, which in fine produces the differentiating light emission with up to 20-fold differences in intensity for the two enantiomers. These findings open up the possibility of developing new ECL-based bioassays and microscopy of chiral environments.
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- Interplay between electrochemistry and optical imaging: The whole is greater than the sum of the parts
- Wireless Anti-Stokes Photoinduced Electrochemiluminescence at Closed Semiconducting Bipolar Electrodes